By Anna Flis, Status:
Purpose: TiMet clock gene expression profiles, baseline comparison across photoperiods in Col wild-type plants, under 6, 8, 12 and 18 h photoperiod. These data define the response of the clock genes under different photoperiods, where other analysis in the TiMet project studied proteome and metabolite levels. Absolute expression levels were compared, as RNA copies per gFW or per cell.
Description: Literature data from: 'Photoperiod-dependent changes in the phase of core clock transcripts and global transcriptional outputs at dawn and dusk in Arabidopsis'
by: Anna Flis.
Col plants were grown in soil under 6, 8, 12 and 18h photoperiod for 21 days, and duplicate samples were harvested at 2 h intervals though a 24 h cycle. qRT-PCR with absolute calibration to spiked-in standards returned RNA copy number per gramme Fresh Weight of rosette tissue. Cell number was separately estimated at 25,000,000 per gFW.
Comments: TiMet experiment WP1.2B.
Data file "TiMet 1.2 - Photoperiod exp - metabolites and transcripts.xlsx;calculations" 2013
Modified by: Andrew Millar on 17-Apr-2016